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After transfer, membranes were blocked in 1% bovine serum albumin in PBS with 0.1% Tween-20 and probed with anti-PARP (NB100-56599, Novus Biologicals, Littleton, CO), anti-Glutathione (AB19534, Abcam, Cambridge, MA), anti-phospho-IB- (9246), anti-IB- (9242), anti-phospho-IKK- (2594), anti-IKK- (2370), or anti-p65 (8242) (Cell Signaling Technology, Danvers, MA) antibodies
doi: 10.1038/s42003-022-03189-z 211 OuwendijkW
One particular group has been investigating metformin as a potential therapeutic intervention to reduce hepatotoxicity after APAP exposure
A vial at day 21 has not suddenly become useless
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